Single-cell RNA sequencing can distinguish cell types that appear identical under a microscope. Because sample preparation removes cells from their original positions, researchers may use a spatial reference map to computationally reconstruct those positions. In one study, a model’s reconstruction closely matched imaging data from the same tissue, a result the researchers presented as evidence that the model was accurate. ______ because those imaging data had also been used to build the model’s reference map, the match did not provide fully independent confirmation of the model.
Which choice completes the text with the most logical transition?